Journal: Analytical chemistry

Article Title: Online 2D-LC-MS/MS Platform for Analysis of Glycated Proteome

doi: 10.1021/acs.analchem.7b03342

Figure Lengend Snippet: Schematic drawing of the online 2D-LC-HCD-MS/MS platform. Boronate affinity enrichment column and C18 column were used as the first and second dimensional separations. The non-glycated peptides (non-binding fraction) were first eluted to waste (valve A with connection in blue); the glycated peptides (binding fraction) were eluted from affinity column to trap column (valve A with connection in red) before eluting to C18 column. A: valve for trapping and RPLC separation of peptides; B: valve for affinity separation of glycated peptides.

Article Snippet: × 1.6 mm o.d.) for self-packed boronate affinity column was purchased from IDEX Health & Science LLC (Oak Harbor, WA).

Techniques: Tandem Mass Spectroscopy, Binding Assay, Affinity Column

Journal: Analytical chemistry

Article Title: Online 2D-LC-MS/MS Platform for Analysis of Glycated Proteome

doi: 10.1021/acs.analchem.7b03342

Figure Lengend Snippet: The chromatograms of boronate affinity enrichment and reversed phase LC separation of glycated peptides from in-vitro glycated human plasma digest (A); the average number of identified peptides and proteins, glycated peptides and proteins from three replicate runs using 1D-LC-HCD-MS/MS without boronate affinity enrichment (1D) and 2D-LC-HCD-MS/MS (2D) (B).

Article Snippet: × 1.6 mm o.d.) for self-packed boronate affinity column was purchased from IDEX Health & Science LLC (Oak Harbor, WA).

Techniques: In Vitro, Tandem Mass Spectroscopy